KORELASI FLAVONOID TOTAL DENGAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAN FRAKSI DAUN PURING KURA (Codiaeum variegatum L.)
DOI:
https://doi.org/10.36423/pharmacoscript.v7i2.1768Keywords:
ANtioksidan, Codiaeum variegatum, flavonoid total, puring kuvaAbstract
Puring kura (Codiaeum variegatum L.) memiliki potensi sebagai antioksidan alami karena kandungan flavonoidnya yang dapat menetralisir radikal bebas dengan cara mendonorkan elektron kepada senyawa radikal bebas. Flavonoid berperan penting dalam aktivitas antioksidan, di mana semakin tinggi konsentrasi flavonoid, semakin besar aktivitas antioksidannya. Penelitian ini bertujuan untuk menentukan korelasi nilai kesetaraan flavonoid total dengan aktivitas antioksidan ekstrak dan fraksi daun puring kura. Ekstrak dan fraksi daun puring kura dipantau menggunakan kromatografi lapis tipis (KLT) dan dilakukan penetapan flavonoid total dan aktivitas antioksidan menggunakan spektrofotometer UV-Vis metode DPPH. Ekstrak etanol, etil asetat, dan n-heksan menunjukkan nilai kesetaraan flavonoid total berturut-turut sebesar 66,690; 96,512 dan 66,184 mg QE/g sampel, sedangkan nilai kesetaraan aktivitas antioksidan adalah 60,109; 33,938 dan 32,836 mg AAE/g sampel. Fraksi gabungan 4 menunjukkan nilai kesetaraan flavonoid paling tinggi sebesar 299,615 mg QE/g sampel, sedangkan nilai kesetaraan aktivitas antioksidan tertinggi dimiliki fraksi gabungan 6 yaitu sebesar 288,599 mg AAE/g sampel. Aktivitas antioksidan ekstrak dan fraksi daun puring kura tidak dipengaruhi oleh kandungan flavonoid total karena pada daun puring kura terdapat senyawa lain yang dapat memberikan aktivitas antioksidan seperti senyawa fenolik lain selain flavonoid atau senyawa-senyawa golongan terpenoid.
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