HYPOGLYCEMIA POTENTIAL OF ETHANOL EXTRACT OF MELINJO LEAVES (Gnetum gnemon L.) ON WISTAR MALE WHITE RATS
DOI:
https://doi.org/10.36423/pharmacoscript.v8i1.2011Keywords:
Alloxan, GOD-PAP, hypoglycemia, melinjo leaves, pancreasAbstract
Male rats were used in the study to evaluate the ethanol extract of melinjo leaf (Gnetum gnemon L.) for hypoglycemia after being given alloxan and having their pancreatic histology observed. Secondary metabolites with antidiabetic properties found in melinjo leaf include flavonoids, tannins, saponins, and phenolics. This study sought to ascertain the ethanol extract of melinjo leaves' ED50 as well as its impact on lowering blood glucose levels and monitoring pancreatic histology. The doses of melinjo leaf ethanol extract were varied to 125, 250, and 500 mg/kgBW. An insulin dosage of 1 IU/kgBW served as the positive control, a 0.5% Na CMC suspension served as the negative control, and a normal control group did not receive any therapy. Rats were tested by utilizing a DTN-410-K photometer to measure their fasting blood glucose levels on days 0, 10, 15, and 20 using the GOD-PAP enzymatic method. Hematoxylin-eosin staining was used in histopathology preparations, which were prepared in accordance with established protocols. Melinjo leaf ethanol extract dosages of 125, 250, and 500 mg/kgBW resulted in three treatment groups with corresponding the percentage of blood glucose reduction (%BGR) of 31.48, 34.39, and 42.90%, whereas the positive control had an average BGR of 40.68%. Melinjo leaf ethanol extract has an ED50 of 720.86 mg/kgBW. According to the histological image, the positive control group and the three treatment groups showed improvement, whereas the negative group's Langerhans islet endocrine cells showed necrosis. The 500 mg/kg BW dosage group shows the greatest improvement.
References
Anisong, N., Siripongvutikorn, S., Wichienchot, S., & Puttarak, P.. (2022). A Comprehensive Review on Nutritional Contents and Functional Properties of Gnetum gnemon Linn.. Food Science and Technology, 42, 1-11. https://doi.org/10.1590/fst.100121
Beroual, K., Agabou, A., Abdeldjelil, M. C., Boutaghane, N., Haouam, S., & Hamdi-Pacha, Y. (2017). Evaluation of Crude Flaxseed (Linum usitatissimum L) Oil in Burn Wound Healing in New Zealand Rabbits. African journal of traditional, complementary, and alternative medicines : AJTCAM, 14(3), 280–286. https://doi.org/10.21010/ajtcam.v14i3.29
Bladt, S. (2009). Plant Drug Analysis: A thin layer chromatography atlas. Springer Science & Business Media. Berlin Heidelberg, New York.
Charisma, A. M. (2017). Korelasi Kadar Rata-Rata Glukosa Darah Puasa dan 2 Jam Post Prondial Tiga Bulan Terakhir Dengan Nilai Hba1c pada Pasien Diabetes Mellitus Prolanis Bpjs Kabupaten Kediri Periode Mei-Agustus 2017. Jurnal Kesehatan Masyarakat Indonesia, 12(2), 1-11.
Depkes RI. (1995). Materia Medika. Departemen Kesehatan Republik Indonesia. Jakarta.
Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Departemen Kesehatan Republik Indonesia. Jakarta.
Edi, D. O. (2020). Potensi Jamur Kuping Hitam (auricularia polytricha) Sebagai Terapi Alternatif Diabetes Melitus. Journal of Health Science and Physiotherapy, 2(1), 9-14. http://dx.doi.org/10.35893/jhsp.v2i1.23
Floris, S., Di Petrillo, A., Pintus, F., & Delogu, G. L. (2024). Pistacia lentiscus: Phytochemistry and Antidiabetic Properties. Nutrients, 16(11), 1638. https://doi.org/10.3390/nu16111638
Gajalakshmi, D., Shettu, N., & Murugesan, S. (2018). Phytochemical Screening of Marine Red Alga Botryocladia leptopoda (J.Agardh) Kylin. International Journal of Interdisciplinary Research and Innovations, 6(2), 463-470.
Handayani, R., & Natasia, G. (2018). Uji Daya Hambat Ekstrak Etanol Daun Sangkareho (Callicarpa longifolia Lam.) terhadap Escherichia coli. Jurnal Surya Medika (JSM), 3(2), 54–61. https://doi.org/10.33084/jsm.v3i2.98
Harborne, J. B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Institut Teknologi Bandung. Bandung.
Hernawati, Kartini, T. A., & Priyandoko, D. (2019). Blood Sugar Conditions in Hyperglycemic Mice After Given the Biscuit from Banana Skin Type Kepok. IOP Conf. Series: Journal of Physics: Conference Series, 1280(022007), 1-5. doi:10.1088/1742-6596/1280/2/022007
Ira, C. D. F., & Cikra. I. (2015). Efek Farmakologi Infusa Biji Melinjo (Gnetum gnemon L.) Sebagai Antihiperglikemia pada Mencit (Mus musculus) yang Diinduksi Dextrosa Monohidrat 40. Journal of Pharmacy Science and Practice, 2(1), 27-32. https://doi.org/10.33508/jfst.v2i1.700
Iriany, Angkasa, H., & Namira, C. A. (2021). Ekstraksi Tanin dari Buah Balakka (Phyllanthus emblica L.) dengan Bantuan Microwave: Pengaruh Daya Microwave, Perbandingan Massa Kering Terhadap Jumlah Pelarut Etil Asetat. Jurnal Sistem Teknik Industri, 10(1), 8-12. https://doi.org/10.32734/jtk.v10i1.5318
Kemenkes RI. (2014). Situasi dan Analisis Diabetes. Kementrian Kesehatan Republik Indonesia. Jakarta.
Kinanti, A., Lestari, A., Nabilah, Z., Maulida, R., Widiastuti, T., & Kiromah, N. (2023). Uji Aktivitas Antidiabetes Ekstrak Etanol Daun Ganitri (Elaeocarpus ganitrus Roxb.) pada Tikus Wistar Jantan (Rattus norvegicus) yang Diinduksi Streptozotocin. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 8(1), 139-151. doi:http://dx.doi.org/10.20961/jpscr.v8i1.64771
Leonita, E., & Muliani, A. (2015). Penggunaan Obat Tradisional oleh Penderita Diabetes Mellitus dan Faktor-faktor yang Berhubungan di Wilayah Kerja Puskesmas Rejosari Pekanbaru Tahun 2015. Jurnal Kesehatan Komunitas, 3(1), 47-52. https://doi.org/10.25311/keskom.Vol3.Iss1.101
Magitasari, H.D., Hidayaturrahmah, H., Santoso, H.B., & Sari, D.K. (2019). Gambaran Histologi Pankreas Tikus Putih (Rattus norvegicus) Hiperglikemia Setelah Pemberian Biskuit Ikan Patin (Pangasius hypopthalmus). Prosiding Seminar Nasional Lingkungan Lahan Basah, 4(1), 211-216.
Mutu, D. A., & Yuda, H. T. (2019). Studi Kasus Penerapan Senam Kaki pada Pasien Diabetes Mellitus di Desa Giwangretno Kabupaten Kebumen. Jurnal Keperawatan Malang, 4(1), 42-48. https://doi.org/10.36916/jkm.v4i1.72
Pertiwi, M. B. B, Indahyani, D. E., & Praharani, D. (2021). Level Glukosa Darah pada Mencit Diabetes Setelah Pemberian Ekstrak Rumput Laut Coklat (Phaeophyta). Pustaka Kesehatan, 9(2), 84-89. doi:10.19184/pk.v9i2.12165
Piero, N. M. et al. (2015). Antidiabetic and Safety of Lantana rhodesiensis in Alloxan Induced Diabetic Rats. Journal of Developing Drugs, 4(1), 1-10. http://dx.doi.org/10.4172/2329-6631.1000129
Puluh, E. A., Edy, H. J., & Siampa, J. P. (2019). Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat. Jurnal Mipa, 8(3), 101-104. https://doi.org/10.35799/jmuo.8.3.2019.25773
Sang, S. V., Elwon, C. A. M., & Iqbal, M. (2023). Total Phenolic, Flavonoid Content, Antioxidant Activity and Phytochemical Screening of Donax grandis (Miq.)Ridl. Borneo International Journal of Biotechnology (BIJB), 3, 63–79. https://doi.org/10.51200/bijb.v3i.4060
Tanamal, M., Papilaya, P., & Smith, A. (2017). Kandungan Senyawa Flavonoid Pada Daun Melinjo (Gnetum gnemon L.) Berdasarkan Perbedaan Tempat Tumbuh. BIOPENDIX: Jurnal Biologi, Pendidikan Dan Terapan, 3(2), 142-147. https://doi.org/10.30598/biopendixvol3issue2page142-147
Ulfa, P.M., Alioes, Y., & Putri, B. (2018). Pengaruh Pemberian Ekstrak Biji Melinjo (Gnetum gnemon) terhadap Kadar Trigliserida pada Tikus dengan Diet Tinggi Lemak. Jurnal Kesehatan Andalas. 7(2), 192-197. http://dx.doi.org/10.25077/jka.v7i2.801
Uno, M.B., Tawas, H.N., & Rate, P.V. (2014). Analisis Kinerja Keuangan, Ukuran Perusahaan, Arus Kas Operasional Pengaruhnya Terhadap Earning Per Share. Jurnal EMBA, 2(3), 745-757. https://doi.org/10.35794/emba.2.3.2014.5656
Wahyuni, Y. S. (2021). Penetapan Kadar Senyawa Terlarut Dalam Pelarut Etanol dan Kadar Air Ekstrak Daun Jambu Mete (Anacardium occidentale L.) Sebagai Parameter Spesifik dan Non Spesifik. Jurnal Kesehatan Yamasi Makassar. 5(1), 105–111.
Widyantari, N.P., & Armita Sari, P.M. (2023). Review: Aktivitas Antioksidan Ekstrak Herba Suruhan (Peperomia pellucida (L.) Kunth). Jurnal Farmasi dan Kesehatan Indonesia, 3(1), 1-3. https://doi.org/10.61179/jfki.v3i1.384
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