QUANTIFICATION OF TOTAL FLAVONOID CONTENT IN FRACTIONATED YOUNG LEAF EXTRACTS OF RED SHOOT LEAVES (Syzygium myrtifolium) USING UV-VIS SPECTROPHOTOMETRY

Syafriana Vilya; Amelia Rika; Maftucha Nissa; Sholikha Munawarohthus; Handayani Windri; Yasman Yasman

doi:10.36423/pharmacoscript.v8i1.2075

Authors

Syafriana Vilya Studi program of Pharmacy, Faculty Phamacy, Institute of Sains dan Teknologi Nasional
Amelia Rika Studi program of Pharmacy, Faculty Phamacy, Institute of Sains dan Teknologi Nasional
Maftucha Nissa Studi program of Pharmacy, Faculty Phamacy, Institute of Sains dan Teknologi Nasional
Sholikha Munawarohthus Studi program of Pharmacy, Faculty Phamacy, Institute of Sains dan Teknologi Nasional
Handayani Windri Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia
Yasman Yasman Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia

DOI:

https://doi.org/10.36423/pharmacoscript.v8i1.2075

Keywords:

ethanol, fractionation, pucuk merah, total flavonoid, young leaf

Abstract

Syzygium myrtifolium (red shoot leaves) is widely known for its rich phytochemical profile, particularly flavonoids, which exhibit various pharmacological properties. This study aimed to determine the total flavonoid content in the crude extract, n-hexane fraction, ethyl acetate fraction, and water fraction of red shoot leaves (Syzygium myrtifolium) using a colorimetric method with quercetin as a standard. The results revealed that the ethyl acetate fraction had the highest flavonoid content (65.781 ± 6.365 mgQE/g), followed by the crude extract (26.093 ± 0.961 mgQE/g), n-hexane fraction (18.293 ± 2.925 mgQE/g), and water fraction (12.583 ± 0.824 mgQE/g). The study also found that the solvent polarity significantly influenced the flavonoid extraction efficiency, with ethyl acetate, a moderately polar solvent, being most effective for isolating flavonoid aglycones. The n-hexane fraction contained polymethyl flavonoids, which are more soluble in nonpolar solvents, while flavonoid glycosides were more soluble in polar solvents like water and alcohol mixtures. Furthermore, the research highlights the importance of extraction methods, as the total flavonoid content in mature red shoot leaves was found to be higher than in younger leaves, likely due to increased secondary metabolite production in mature tissues. This study emphasizes the role of solvent polarity in flavonoid extraction and provides insights into the phytochemical composition of S. myrtifolium leaves for potential applications in natural product research.

References

Abubakar, A. R., & Haque, M. (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences, 12(1), 1–10.

Ahmad, M. A., Lim, Y. H., Chan, Y. S., Hsu, C.-Y., Wu, T.-Y., & Sit, N. W. (2022). Chemical Composition, Antioxidant, Antimicrobial and Antiviral Activities of the Leaf Extracts of Syzygium myrtifolium. Acta Pharmaceutica, 72(2).

Andersen, O. M., & Markham, K. R. (2005). Flavonoid: Chemistry, Biochemistry and Applications. CRC Press.

Anggraini, T. (2017). Antioxidant Activity of Syzygium oleana. Pakistan Journal of Nutrition, 16(8), 605–611.

Azzahra, F., & Budiati, T. (2022). Pengaruh Metode Pengeringan dan Pelarut Ekstraksi terhadap Rendemen dan Kandungan Kimia Ekstrak Daun Alpukat (Persea americana Mill.). Medical Sains : Jurnal Ilmiah Kefarmasian, 7(1), 67–78.

Bangun, P. P. A., Rahman, A. P., & H., S. (2021). Analisis Kadar Total Flavonoid pada Daun dan Biji Pepaya (Carica papaya L .) Menggunakan Metode spektrofotometer UV-vis. Jurnal Ilmiah Farmasi Attamru.

Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10(3), 178–182.

Depkes RI. (1995). Materia Medika Indonesia. Direktorat Jenderal Pengawasan Obat dan Makanan.

Dias, Ma. C., Pinto, D. C. G. A., & Silva, A. M. S. (2021). Plant Flavonoids : Chemical Characteristics and Biological Activity. Molecules, 26(17).

Doloking, H., Mukhriani, Ningsi, S., & Tahar, N. (2022). Flavonoids: A Review on Extraction, Identification, Quantification, and Antioxidant Activity. Ad-Dawaa' Journal of Pharmaceutical Sciences, 5(1), 1-26.

Fadillah, A., Rahmadani, A., & Rijai, L. (2017). Analisis Kadar Total Flavonoid dan Uji Aktivitas Antioksida Ekstrak Daun Kelubut (Passiflora foetida L.). Proceeding of the 5th Mulawarman Pharmaceuticals Conferences, 23–24.

Fadiyah, I., Lestari, I., Victory, S., & Mahardika, R. G. (2019). Uji Aktivitas Antioksidan dari Ekstrak Maserasi Buah Rukam (Flacourtia Rukam). Jurnal Sains Dan Terapan Kimia, 1(1), 14–19.

Febriani, D., Salni, & Marisa, H. (2022). Aktivitas senyawa antioksidan daun Godobos (Enhydra fluctuans Lour.). Sriwijaya Bioscientia, 3(2), 40-48.

Harborne, J. B. (1987). Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. Penerbit ITB.

Haryati, N. A., Saleh, C., & Erwin. (2015). Uji Toksisitas dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Kimia Mulawarman, 13(1), 35–40.

Irianti, T. T., Kuswandi, Nuranto, S., & Purwanto. (2021). Antioksidan dan Kesehatan. Gadjah Mada University Press.

Kemenkes RI. (2017). Farmakope Herbal Indonesia. Kementrian Kesehatan RI.

Koirala, N., Huy, N., Prasad, G., Thang, D. Van, & Kyung, J. (2016). Enzyme and Microbial Technology Methylation of flavonoids : Chemical structures, bioactivities, progress and perspectives for biotechnological production. Enzyme and Microbial Technology, 86, 103–116. https://doi.org/10.1016/j.enzmictec.2016.02.003

Kumaradewi, D. A. P., Subaidah, W. A., Andayani, Y., & Al-Mokaram, A. (2021). Phytochemical Screening And Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L . Spreng) Using DPPH Method. Jurnal Penelitian Pendidikan IPA, 7(2). https://doi.org/10.29303/jppipa.v7i2.675

Kusriani, R. H., Rosandhy, S. M., & Elfahmi. (2019). Luteolin , a Flavonoid from Syzygium myrtifolium Walp. Current Research on Biosciences and Biotechnology, 1(1), 31–33.

Kusuma, C.A., Nastiti, K., & Budi, S. (2022). Identifikasi Senyawa Kimia dan Penetapan Kadar Flavonoid Total Pada Tingkatan Fraksi Daun Hapa-hapa (Flemingia macrophylla). Sains Medisina, 1(2), 83-90.

Lindawati, N. Y., & Ma’ruf, S. H. (2020). Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus vulgaris L.) dengan Metode Kompleks Kolorimetri secara Spektrofotometri Visibel. Jurnal Ilmiah Manuntung, 6(1), 83–91.

Lubis, S. M., Siregar, P., & Lenny, S. (2020). Isolation of Flavonoid Compounds from Guava Plant Leaves (Syzygium malaccense (L.) Merr. & Perry). Journal of Chemical Natural Resources, 02(02), 132–140.

Markham, K. R. (1988). Cara Mengidentifikasi Flavonoid. ITB Press.

Memon, A. H., Ismail, Z., Aisha, A. F. A., Al-Suede, F. S. R., Hamil, M. S. R., Hashim, S., Saeed, M. A. A., Laghari, M., & Abdul Majid, A. M. S. (2014). Isolation, characterization, crystal structure elucidation, and anticancer study of dimethyl cardamonin, isolated from Syzygium campanulatum Korth. Evidence-Based Complementary and Alternative Medicine, 1–12. https://doi.org/10.1155/2014/470179

Najib, A. (2018). Ekstraksi Senyawa Bahan Alam. Deepublish.

Ningsih, D. R. (2017). Ekstrak Daun Mangga (Mangifera indica L.) sebagai Antijamur terhadap Jamur Candida Albicans dan Identifikasi Golongan Senyawanya. Jurnal Kimia Riset, 2(1), 61.

Nofita, D., Sari, S. N., & Mardiah, H. (2020). Penentuan Fenolik Total dan Flavonoid Ekstrak Etanol Kulit Batang Matoa (Pometia pinnata J.R & G.Forst) secara Spektrofotometri. Chimica et Natura Acta, 8(1), 36–41.

Novi, C., Aisah, S., Dita, L., Kartika, E. Y., Endrawati, S., & Susilo, H. (2023). Formulasi dan Uji Aktivitas Sediaan Gel Ekstrak Daun Kacapiring (Gardenia Jasminodes J.Ellis) terhadap Bakteri Staphylococcus epidermidis. J-medSains, 3(1), 35-45.

Nugroho, A. (2017). Buku Ajar Teknologi Bahan Alam. Banjarmasin: Lambung Mangkurat University Press.

Nurlinda, Handayani, V., & Rasyid, F. A. (2021). Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L .) Leaves. Jurnal Fitofarmaka Indonesia, 8(3), 1–4.

Panaungi, A.N., & Sakka, L. (2022). Pelatihan Pembuatan Simplisia Daun Kelor (Morinaga oliefera) Pada Masyarakat Desa Mangeloreng Kecamatan Bantimurung, Kabupaten Maros. Jurnal Pengabdian Farmasi dan Sains (JPFS), 01(01), 36-39.

Pangondian, A., Athaillah, Chandra, P., & Renaldi, R. (2023). Edukasi Pemanfaatan Pengawetan Bahan Alam dengan Metode Simplisia pada Siswa SMP Pahlawan Medan. Jukeshum: Jurnal Pengabdian Masyarakat, 3(2), 291-295.

Parbuntari, H., Prestica, Y., Gunawan, R., Nurman, M. N., & Adella, F. (2018). Preliminary Phytochemical Screening of Cacao Leaves (Theobroma cacao L.). EKSAKTA: Berkala Ilmiah Bidang MIPA, 19(2), 40–45.

Priamsari, M. R., Susanti, M. M., & Atmaja, A.H. (2016). Pengaruh Metode Pengeringan Terhadap Kualitas Ekstrak dan Kadar Flavonoid Total Ekstrak Etanolik Daun Sambung Nyawa (Gynura Procumbens (Lour.) Merr.). Jurnal Farmasi, 5(1), 29-33.

Santoni, A., Darwis, D., & Syahri, S. (2013). Isolasi Antosianin dari Buah Pucuk Merah (Syzygium campanulatum korth.) serta Pengujian Antioksidan dan Aplikasi sebagai Pewarna Alami. PRosiding Semirata FMIPA Universitas Lampung, 1–10.

Shraim, A. M., Ahmed, T. A., Rahman, M., & Hijji, Y. M. (2021). Determination of Total Flavonoid Content by Aluminum Chloride Assay : A Critical Evaluation. LWT, 150, 111932. https://doi.org/10.1016/j.lwt.2021.111932

Suharyanto, S., & Hayati, T. N. (2021). Penetapan Kadar Flavonoid Total Ekstrak Buah Gambas (Luffa acutangula (L.) Roxb.) dengan Metode Spektrofotometri UV-Vis. Pharmacon: Jurnal Farmasi Indonesia, 18(1), 82–88.

Sukmawati, Sudewi, S., & Pontoh, J. (2018). Optimasi dan Validasi Metode Analisis dalam Penentuan Kandungan Total Flavonoid pada Ekstrak Daun Gedi Hijau (Abelmoscus manihot L.) yang Diukur Menggunakan Spektrofotometer UV-VIS. Pharmacon, 7(3), 32–41.

Sunarti. (2021). Daun Pucuk Merah : Inovasi dan Pengembangan Obat Herbal sebagai Terapi Antidiabetes. Literasi Nusantara.

Susanti, D. & Safrina, D. (2021). Analisis Faktor Internal Tenaga kerja yang Mempengaruhi Kecepatan dan Ketelitian Sortasi Basah Tanaman Pegangan. Agrointek, 15(1), 25-34.

Syafriana, V., & Wiranti, Y. (2022). Potensi Daun Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) sebagai Agen Antibakteri terhadap Streptococcus mutans. Farmasains, 9(2), 65–75.

Syafriana, V., Febriani, A., Suyatno, S., Nurfitri, N., & Hamida, F. (2021). Antimicrobial Activity of Ethanolic Extract of Sempur (Dillenia suffruticosa (Griff.) Martelli) Leaves against Pathogenic Microorganisms. Borneo Journal of Pharmacy, 4(2), 135–144.

Tanaya, V., Retnowati, R., & Suratmo. (2015). Fraksi Semipolar dari Daun Mangga Kasturi (Mangifera casturi Kosterm). Kimia Student Journal, 1(1), 778-784.

Tandi, J., Melinda, B., Purwantari, A., & Widodo, A. (2020). Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L . Moench ) dengan Metode Spektrofotometri UV-Vis [Qualitative and Quantitative Analysis of Secondary Metabolites in Ethanol Extract of Okra (Abelm. Kovalen: Jurnal Riset Kimia, 6(April), 74–80.

Wahyusi, K. N., Irmawati, N. D., & Astari, R. Z. (2020). Koefisien Perpindahan Massa Ekstraksi Flavonoid Dari Buah Pare Dengan Pelarut Etanol. Jurnal Teknik Kimia, 14(2), 40–44.

Wen, L., Jiang, Y., Yang, J., Zhao, Y., Tian, M., & Yang, B. (2017). Structure, Bioactivity, and Synthesis of Methylated Flavonoids. Annals of the New York Academy of Sciences, 1398(1), 120–129. https://doi.org/10.1111/nyas.13350

Wijaya, A., Widiastuti, N. R., & Rahmadani, A. N. (2023). Aktivitas Antioksidan Fraksi Air, Etil Asetat, dan Kloroform Ekstrak Daun Pepaya (Carica papaya L.) dengan Metode DPPH. Jurnal Jamu Kusuma, 3(2), 62-68.

Winahyu, D. A., Retnaningsih, A., & Aprillia, M. (2019). Penetapan Kadar Flavonoid pada Kulit Batang Kayu Raru (CotylelobiummelanoxylonP) dengan Metode Spektrofotometri UV-Vis. Jurnal Analis Farmasi, 4(1), 29–36.