DESAIN VAKSIN MULTI-EPITOP TERHADAP VIRUS MARBURG (MARV) BERBASIS PROTEIN VP35 MENGGUNAKAN PENDEKATAN IMMUNOINFORMATIKA: IN-SILICO

Asopari Ilham Nurfadilah; Pratita Anindita Tri Kusuma; Mardianingrum Richa; Ruswanto Ruswanto

doi:10.36423/pharmacoscript.v8i2.2184

Authors

Asopari Ilham Nurfadilah Program Studi Farmasi, Fakultas Farmasi, Universitas Bakti Tunas Husada Tasikmalaya
Pratita Anindita Tri Kusuma Program Studi Farmasi, Fakultas Farmasi, Universitas Bakti Tunas Husada Tasikmalaya
Mardianingrum Richa Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Perjuangan, Tasikmalaya
Ruswanto Ruswanto Program Studi Farmasi, Fakultas Farmasi, Universitas Bakti Tunas Husada Tasikmalaya (ID Scopus:57194336756)

DOI:

https://doi.org/10.36423/pharmacoscript.v8i2.2184

Keywords:

Virus Marburg, Immunoinformatika, Bioinformatika, VP35, Desain Vaksin

Abstract

Virus Marburg (MARV) merupakan salah satu virus zoonosis dari keluarga Filoviridae dengan tingkat kematian hingga 88%. Pertumbuhan populasi global yang pesat meningkatkan risiko paparan penyakit akibat perubahan iklim dan ekologi. Untuk pencegahan, vaksin menjadi upaya terbaik. Penelitian ini bertujuan merancang vaksin berbasis peptida dari protein VP35 dan mengevaluasi interaksinya dengan sistem imun (TLR3, MHC-I, dan MHC-II) berdasarkan parameter stabilitas interaksi molekuler seperti skor ikatan, nilai elektrostatik, dan desolvasi. Metode yang digunakan adalah pendekatan immunoinformatics secara in silico, meliputi pemilihan protein target, prediksi antigenisitas, prediksi epitop T CD8+, prediksi epitop T CD4+, prediksi epitop B, konstruksi vaksin & visualisasi 3D, validasi struktur vaksin, analisis docking dan prediksi cakupan populasi. Hasil menunjukkan bahwa desain vaksin memiliki interaksi yang stabil terhadap TLR3, MHC-I (HLA-A*11:01), dan MHC-II (HLA-DR1), dengan nilai skor total berturut-turut sebesar –43,698, –42,192, dan –51,899, menunjukkan afinitas ikatan yang kuat secara in silico. Temuan ini mengindikasikan potensi desain vaksin berbasis epitop VP35 sebagai kandidat imunogenik terhadap MARV, meskipun perlu dioptimalkan lebih lanjut mengingat cakupan populasi yang masih terbatas.

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