DETERMINATION OF SHELF LIFE USING ACCELERATED SHELF LIFE TEST (ASLT) METHOD ON INSTANT POWDER PROBIOTIC ORANGE SWEET POTATO FREEZE DRY
DOI:
https://doi.org/10.36423/pharmacoscript.v9i1.2697Keywords:
Shelf Life, Accelerated Shelf Life Testing, Orange Sweet Potato, Freeze Drying, Instant Drink PowderAbstract
Probiotic beverages produced using freeze-drying techniques are commonly developed to extend shelf life. In this study, instant probiotic powder made from orange sweet potato was prepared using the freeze-drying process at -56°C with the addition of 20% maltodextrin as a coating material. This study aimed to determine the shelf life of instant orange sweet potato probiotic powder using the Accelerated Shelf Life Testing (ASLT) method based on the Arrhenius equation model. The instant orange sweet potato probiotic powder was stored at temperatures of 4°C, 28°C, and 40°C for 56 days. The parameters observed every seven days were moisture content, pH, total acidity, and total lactic acid bacteria (LAB). The experimental results showed a linear relationship between the increase in moisture content and pH, and the decrease in total acidity and total LAB count over storage time at each temperature. The estimated shelf life of the instant orange sweet potato probiotic powder stored at 4°C, 28°C, and 40°C was 49, 45, and 43 days based on moisture content; 34, 42, and 46 days based on pH; 212, 207, and 206 days based on total acidity; and 236, 306, and 344 days based on total LAB, respectively. The shelf life of instant probiotic orange sweet potato powder can be determined using the ASLT method, with variations depending on the quality parameters evaluated.
References
Bottiroli, R. et al. (2021) ‘Understanding the effect of storage temperature on the quality of semi-skimmed UHT hydrolyzed-lactose milk: An insight on release of free amino acids, formation of volatiles organic compounds and browning’, Food Research International, 141, p. 110120. doi:10.1016/j.foodres.2021.110120.
Chaturvedi, S. and Chakraborty, S. (2024) ‘Effect of temperature and packaging materials on the shelf-life stability and in vitro properties of microencapsulated and spray-dried synbiotic legume-based instant beverage powder’, Sustainable Food Technology, 2(1), pp. 162–174. doi:10.1039/D3FB00094J.
Daysita, L.E. et al. (2024) ‘Characterization and shelf life of synbiotic drink powder from porang (Amorphophallus muelleri)’, Journal of Food Science and Technology, 61(7), pp. 1272–1282. doi:10.1177/10820132211020621.
Dianawati, D., Mishra, V. and Shah, N.P. (2016) ‘Survival of microencapsulated probiotic bacteria after processing and during storage: a review’, Critical reviews in food science and nutrition, 56(10), pp. 1685–1716. doi:10.1080/10408398.2013.798779.
Estiasih, T., Waziiroh, E. and Fibrianto, K. (2022) Kimia dan Fisik Pangan. Bumi Aksara.
Ferdaus, F. et al. (2017) ‘Pengaruh pH, konsentrasi substrat, penambahan kalsium karbonat dan waktu fermentasi terhadap perolehan asam laktat dari kulit pisang’, Widya Teknik, 7(1), pp. 1–14.
Gänzle, M.G. (2015) ‘Lactic metabolism revisited: metabolism of lactic acid bacteria in food fermentations and food spoilage’, Current Opinion in Food Science, 2, pp. 106–117. doi:10.1016/j.cofs.2015.03.001.
Gullifa, G. et al. (2023) ‘Microencapsulation by a Spray Drying Approach to Produce Innovative Probiotics-Based Products Extending the Shelf-Life in Non-Refrigerated Conditions’, Molecules, 28(2), p. 860. doi:10.3390/molecules28020860.
Miskiyah, J. and Yuanita, L. (2020) ‘Quality of Probiotic Yogurt Dry Starter at Various Temperatures During Storage’, Jurnal Penelitian Pascapanen Pertanian, 17(1), pp. 15–23.
Nasional, B.S. (2009) ‘Yoghurt’, Standar Nasional Indonesia. SNI, 2981, p. 2009.
Pradipta, M.S.I. (2018) ‘The Effect of Microencapsulation of Indigenous Poultry Lactic Acid Bacteria Probiotics Using Maltodextrin Coating Material on Viability During Heating’, Journal of Livestock Science and Production, 2(1), pp. 73–78.
Putri, A.N., Kusnadi, I.J. and Saparianti, E. (2021) ‘Innovation in Fermented Normal Yogurt and Caspian Sea Yogurt Drinks Using Various Herbal Plants’. Universitas Brawijaya.
Rossi, E. et al. (2021) ‘Physicochemical and microbiological properties of yogurt made with microencapsulation probiotic starter during cold storage’, Biodiversitas Journal of Biological Diversity, 22(4).
Sukarminah, E. et al. (2017) ‘The Effect Of Sorgum Flow Concentration (Sorghum Bicolor L. Moench) On Some Characteristics Of Sinbiotic Drinks Donggala, Sulawesi Tengah Pr’, Asian Journal of Environment, History and Heritage, 1(2).
Vivek, K., Mishra, S. and Pradhan, R.C. (2021) ‘Optimization of spray drying conditions for developing nondairy based probiotic sohiong fruit powder’, International Journal of Fruit Science, 21(1), pp. 193–204.
Yulia, N., Rubiyanti, R. and Yuliana, A. (2024) ‘Formulation and Characterization of Instant Probiotic Powder from Orange Sweet Potato (Ipomoea batatas L) Using Lactobacillus bulgaricus and Streptococcus thermophilus Bacterial Cultures’, Acta Scientific Microbiology, 7(January 01, 2024), pp. 34–40.
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