THE ETHANOL EXTRACT OF Piper sarmentosum Roxb. IMPROVES METABOLIC PARAMETERS IN HIGH-FAT DIET-INDUCED MICE

Khoiroh Itsna Rofiidatul; Levita Jutti; Sumiwi Sri Adi

doi:10.36423/pharmacoscript.v9i2.2851

Authors

Khoiroh Itsna Rofiidatul Master Program in Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
Levita Jutti Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
Sumiwi Sri Adi Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia

DOI:

https://doi.org/10.36423/pharmacoscript.v9i2.2851

Keywords:

Diabetes Mellitus, Flavonoids, Insulin Resistance, Phenolics, Piperaceae, Piper sarmentosum

Abstract

A high-fat diet (HFD) is a major risk factor for insulin resistance (IR), obesity, and metabolic disorders. Flavonoids have been reported to attenuate IR and obesity-related metabolic alterations in experimental animal models. As Piper sarmentosum Roxb. leaves are rich in flavonoids and phenolic compounds, this study aimed to evaluate the effects of the ethanol extract of P. sarmentosum leaves (EEPS) on body weight, abdominal circumference, and liver index in HFD-fed mice. Mice were fed an HFD to induce IR and treated with EEPS at different doses. EEPS at 250 mg/kg significantly attenuated HFD-induced increases in body weight gain (12.80%), abdominal circumference (8.44%), and liver index (6.87%) compared with the negative control group (p < 0.05). These findings suggest that EEPS attenuates HFD-induced increases in body weight, abdominal adiposity, and liver index in mice.

References

Abbasi, E., & Khodadadi, I. (2025). High-fat diet may increase the risk of insulin resistance by inducing dysbiosis. Metabolism Open, 27, 100381. https://doi.org/10.1016/j.metop.2025.100381

Al-Kuraishy, H. M., Al-Gareeb, A. I., Alexiou, A., Papadakis, M., Nadwa, E. H., Albogami, S. M., Alorabi, M., Saad, H. M., & Batiha, G. E. (2022). Metformin and growth differentiation factor 15 (GDF15) in type 2 diabetes mellitus: A hidden treasure. Journal of Diabetes, 14(12), 806–814. https://doi.org/10.1111/1753-0407.13334

Azelan, A., Taher, Z. M., Sasano, S., Ariga, T., & Aziz, A. A. (2020). Chemical constituents and bioactivity of Piper sarmentosum: A Mini Review. Food Research, 4, 14–18. https://doi.org/10.26656/fr.2017.4(S2).S10

Bilbina, A., Zurriyani, Z., & Feriyani, F. (2024). Hubungan antara lingkar perut dengan diabetes melitus pada pasien Poli Penyakit Dalam di RS Meuraxa. Media Kesehatan Masyarakat Indonesia, 23(1), 18-23. https://doi.org/10.14710/mkmi.23.1.18-23

Chen, J., Xiao, Y., Li, D., Zhang, S., Wu, Y., Zhang, Q., & Bai, W. (2023). New insights into the mechanisms of high-fat diet-mediated gut microbiota in chronic diseases. iMeta, 2(1), e69. https://doi.org/10.1002/imt2.69

Dedual, Mara A., Wueest, Stephan., Borsigova, Marcela., Konrad, Daniel. (2019). Intermittent fasting improves metabolic flexibility in short-term high-fat diet-fed mice. American Journal of Physiology-Endocrinology and Metabolism, 317:5, E773-E782.

https://doi.org/10.1152/ajpendo.00187.2019

Deng, M., Li, Z., Chen, S., Wang, H., Sun,

L, Tang, J., Luo, L., Zhang, X., Xu, H., & Dai, Z. (2024). Exploring the heterogeneity of hepatic and pancreatic fat deposition in obesity: implications for metabolic health. Frontiers in Endocrinology, 15, 1447750. https://doi.org/10.3389/fendo.2024.1447750

International Diabetes Federation. (2025). IDF Diabetes Atlas (11th ed.). International Diabetes Federation. https://diabetesatlas.org

Kementerian Kesehatan Republik Indonesia. 2017. Farmakope Herbal Indonesia Edisi II.

Kumar, S. R., Mohd Ramli, E. S., Abdul Nasir, N. A., Mohd Ismail, N., & Mohd Fahami, N. A. (2021). Methanolic Extract of Piper sarmentosum Attenuates Obesity and Hyperlipidemia in Fructose-Induced Metabolic Syndrome Rats. Molecules (Basel, Switzerland), 26(13), 3985. https://doi.org/10.3390/molecules26133985

Lema, A. E., Ningsih, D., & Nopiyanti, V. (2015). Aktivitas antihiperglikemik ekstrak air daun sukun (Artocarpus altilis (Park.) Fosberg) terhadap tikus diabetes yang diinduksi aloksan. J Farm Indones, 12, 94-101.

Nguyen, N. Q., Nguyen, V. T., Van, N. T. H., & Vo., T. N. M. (2020). Bioactive compounds and antioxidant activity of leaves from Piper sarmentosum Piperaceae. IOP Conference Series: Materials Science and Engineering 991(1). https://doi.org/10.1088/1757899X/91/1/012028

Sui, X., Liu, Y., Zhao, J., Wang, Z., & Zhang, G. (2025). Dietary flavonoids may improve insulin resistance: NHANES, network pharmacological analyses and in vitro experiments. PloS one, 20(12), e0338100. https://doi.org/10.1371/journal.pone.0338100

Sukandar, E. Y., Qowiyyah, A., & Larasari, L. (2011). Effect of methanol extract hearhleaf madeiravine (Anredera Cordifolia (Ten.) Steenis) leaves on blood sugar in diabetes mellitus model mice. Jurnal Medika Planta, 1(4), 1-10.

Verkouter, I., Noordam, R., le Cessie, S., van Dam, R. M., Lamb, H. J., Rosendaal, F. R., van Heemst, D., & de Mutsert, R. (2019). The Association between Adult Weight Gain and Insulin Resistance at Middle Age: Mediation by Visceral Fat and Liver Fat. Journal of Clinical Medicine, 8(10), 1559. https://doi.org/10.3390/jcm8101559

Yan K. (2024). Recent advances in the effect of adipose tissue inflammation on insulin resistance. Cellular signalling, 120, 111229. https://doi.org/10.1016/j.cellsig.2024.111229

Zhao, X., An, X., Yang, C., Sun, W., Ji, H., & Lian, F. (2023). The crucial role and mechanism of insulin resistance in metabolic disease. Frontiers in endocrinology, 14, 1149239. https://doi.org/10.3389/fendo.2023.1149239