In Vitro Conservation of Medicinal Plant St. John’s Wort (Hypericum perforatum L.) Through Dilution of Basic Medium
DOI:
https://doi.org/10.36423/agroscript.v3i1.589Abstract
In vitro techniques could be applied for plant conservation through minimal growth. Growth reduction is generally attained by modifying the culture medium and/or the environmental condition. Conservation of St. John’s wort (Hypericum perforatum L.) in vitro through dilution of basic medium was conducted at the Tissue Culture Laboratory of Indonesian Spice and Medicinal Crops Research Institute (ISMCRI) in 2018. Sterile shoots were cultured into Murashige and Skoog (MS) medium by reducing macronutrients. The treatments used were full-MS + 0.1 mg L-1 N6 - benzyladenine (BA) as control; ¾ MS + 0.1 mg L-1 BA; ½ MS + 0.1 mgL-1 BA and ¼ MS + 0.1 mg L-1 BA. Observation of the culture growth was conducted three months after the treatments. The experiment was arranged in Completely Randomized Design with ten replications. The result showed that the use of a dilution of basic medium affected the growth of St. John’s wort. The use of ¼ MS + 0.1 mg L-1 BA composition could suppress culture growth (number of shoots, shoots length and number of leaves), without showing necrotic symptom until three months of conservation. This treatment could be used as an alternative to minimize the culture of St. John’s wort for in vitro conservation.
References
Arumugam, M., Lulu, S., Kumari, S, & Kumari, N. V. D. (2013). Computational screening and evaluation of bioactive compounds against NS3 helicase of HCV. Int. J. Pharm. Pharm Science 5(2013), 370-376.
Barnes, J, Anderson, L. A, & Phillipson, J. D. (2001). St John’s wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J. Pharm Pharmacology 53(5), 583-600.
Chawala, H. S. (2002). Introduction to plant biotechnology. Science Publishers. ISBN.9781578082285. DOI:10.1201/9781315275369.
Dube, P., Gangopadhyay, M., Dewanjee, S, & Nasim, A. M. (2011). Establishment of a rapid multiplication protocol of Coleus Forskohlii Briq and in vitro conservation by reduced growth. Indian Journal of Biotechnology. 10(2011), 228-231.
Ebadi, M. (2002). Pharmacodynamic basis of herbal medicine 36. Hypericum (St. John’s Wort and Depression). CRC Press. Washington, 726p.
Engelmann, F, & Engels, J. M. M. (2002). Technologies and strategies for ex situ conservation. pp. 89-104, in: JHH Engels, V.R. Rao, AH.D. Brown and M.T. Jackson (editors). Managing Plant Genetic Diversity. CABI, Wallingford, UK, & IPGRI, Rome, Italy.
Engelmann, F. (2011). Use of biotechnologies for the conservation of plants biodiversity. In vitro Cell Dev. Biol. Plant 47(2011), 5-16.
Lat, H., Rita, M. M., Bianca, B., Ana, M, & Pereira, S. (2010). In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In vitro cellular & Developmental Biology-Plant. 4691(2010), 22-27.
Lopez-Puc, G. (2013). An Effective in vitro slow growth protocol for conservation of the orchid Ephidendrum clorocorymbos Schltr. Tropical and Sub Tropical Agroecosystem 16(2013), 61-68.
Monteiro, A. C. B., Higashi, E. N., Gonçalves, A. N., & Rodriguez, A. P. M. (2000). A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis sims. flavicarpa deg.). In Vitro Cell. Dev. Biol. Plant 36(2000), 527-531.
Morshedloo, M., Ebadi, A., Fatahi, M., & Yazdani, D. (2012). Evaluation of essential oil composition in tree species of Hypericum from Iran. J Med Plants 11(9), 23–31.
Penjweini, R., Loew, H. G., Breit, P., & Kratky, K. W. (2013). Optimizing the antitumor selectivity of PVP-Hypericin re A549 cancer cells and HLF normal cells through pulsed blue light. Photodiagnosis Photodyn Ther 10 (4), 591-599. doi:10.1016/j.pdpdt.2013.06.005.
Rajasekharan, P. E, & Sahijram, L. (2015). In Vitro Conservation of Plant Germplasm. Plant Biology and Biotechnology: Volume II: Plant Genomics and Biotechnology. DOI. 10.1007/978-81-322-2283-5_22.
Skyba, M., Faltus, M., Zamecnik, J., & Cellarova, E. (2011). Thermal analysis of cryopreserved Hypericum perforatum L shoot tips: cooling regime dependent dehydration and ice growth. Thermochimica Acta. 514 (1-2), 22-27. DOI.10.1016/j.tca.2010.11.027.
Sota, V, & Kongjika, E. (2014). The effect of nutrient media in micropropagation and in vitro conservation of wild population of Mahaleb cherry (Prunus mahaleb L.). Journal of Microbiology, Biotechnology and Food Sciences, 3(6), 453-456.
Syahid, S. F, & Bermawie, N. (2000). Pengaruh pengenceran media dasar terhadap pertumbuhan kultur jahe dalam penyimpanan secara in vitro. Jurnal Penelitian Tanaman Industri, 594 (2000),115-118.
Syahid, S. F, & Wahyuni, S. (2019). Effect of silver nitrate on shoots multiplication, rooting induction and plantlets characteristics of St. john’s wort (Hypericum perforatum L.) in vitro culture. African Journal of Agricultural Research, 14(27), 1149-1153.
Downloads
Published
How to Cite
Issue
Section
License
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be transferred to AGROSCRIPT Journal of Applied Agricultural Sciences
This is an open-access journal in accordance with the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license.
