Pertumbuhan Planlet Anggrek Papilionanthe hookeriana x Vanda limbata yang Diinokulasikan Trichoderma sp. pada Fase Aklimatisasi
DOI:
https://doi.org/10.36423/agroscript.v5i2.1265Abstract
Anggrek termasuk dalam komoditas pertanian yang memiliki nilai ekonomi yang tinggi. Tahap aklimatisasi merupakan tahap yang menentukan keberhasilan proses adaptasi anggrek. Tujuan penelitian ini untuk pengetahui pengaruh Trichoderma sp. terhadap daya hidup dan pertumbuhan planlet pada tahap aklimatisasi. Percobaan ini menggunakan Rancangan Acak Kelompok Lengkap dengan dua faktor, yaitu dosis Trichoderma sp. dan konsentrasi bakterisida. Konsentrasi bakterisida berpengaruh terhadap persentase hidup planlet. Hasil penelitian menunjukkan bahwa konsentrasi bakterisida 3 g/L dapat meningkatkan persentase hidup planlet hingga 87,50%. Dosis Trichoderma sp. 2,50 g berpengaruh pada peningkatan tinggi tanaman hingga 9,77 cm. Namun, dosis Trichoderma sp. dan bakterisida tidak berpengaruh terhadap jumlah akar, panjang akar, jumlah daun, dan bobot basah tanaman.
References
Abd-El-Khair, H., Elshahawy, I. E., & Haggag, H. E. K. (2019). Field application of Trichoderma spp. combined with thiophanate-methyl for controlling Fusarium solani and Fusarium oxysporum in dry bean. Bulletin of the National Research Centre, 43(1). Retrieved from: https://doi.org/10.1186/s42269-019-0062-5.
Billore, V., Mirajkar, S. J., Suprasanna, P., & Jain, M. (2019). Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid. Biotechnology Reports, 22, e00343. Retrieved from: https://doi.org/10.1016/j.btre.2019.e00343.
Brown, D. M., Groom, C. L., Cvitanik, M., Brown, M., Cooper, J. L., & Arditti, J. (1981). Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro. Plant Cell, Tissue and Organ Culture, 1(1), 165–180. Retrieved from: https://doi.org/10.1007/BF02318914.
Cardoso, J. C. (2017). Ionocidium ‘Cerrado 101’: intergeneric orchid hybrid with high quality of blooming. Ornamental Horticulture, 23(3), 351–356. Retrieved from: https://doi.org/10.14295/oh.v23i3.1110.
Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R. (2010). Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnology Letters, 32(9), 1199–1205. Retrieved from: https://doi.org/10.1007/s10529-010-0290-0.
Devadas, R., Pattanayak, S. L., & Singh, D. R. (2016). Studies on cross compatibility in Dendrobium species and hybrids. Indian Journal of Genetics and Plant Breeding, 76(3), 344–355. Retrieved from: https://doi.org/10.5958/0975-6906.2016.00052.3.
Fatmawati, E., & Rahmawati, F. (2020). Aklimatisasi tembakau (Nicotiana tabaccum L.) pada media kompos cocopeat dan kotoran kambing. Agropross: National Conference Proceedings of Agriculture, 4(2020), 26–32. Retrieved from: https://doi.org/10.25047/agropross.2020.8
Gutiérrez-Miceli, F. A., Ayora-Talavera, T., Abud-Archila, M., Salvador-Figueroa, M., Adriano-Anaya, L., Arias Hernández, M. L., & Dendooven, L. (2008). Acclimatization of micropropagated orchid Guarianthe skinnerii inoculated with Trichoderma harzianum. Asian Journal of Plant Sciences, 7(3), 327–330. Retrieved from: https://doi.org/10.3923/ajps.2008.327.330.
Indravathi, G., & Babu, P. S. (2019). Enhancing acclimatization of tissue cultured plants of Albizia amara by Biotization. International Journal of Scientific Research in Biological Sciences, 6(4), 43–50. Retrieved from: https://doi.org/10.26438/ijsrbs/v6i4.4350.
Kamal, R. K. (2018). Trichoderma: a most common biofertilizer with multiple roles in agriculture. Biomedical Journal of Scientific & Technical Research, 4(5), 9–11. Retrieved from: https://doi.org/10.26717/bjstr.2018.04.0001107.
Khan, R. A. A., Najeeb, S., Hussain, S., Xie, B., & Li, Y. (2020). Bioactive secondary metabolites from Trichoderma spp. Against phytopathogenic fungi. Microorganisms, 8(6), 1–22. Retrieved from: https://doi.org/10.3390/microorganisms8060817.
Nieto-Jacobo, M. F., Steyaert, J. M., Salazar-Badillo, F. B., Vi Nguyen, D., Rostás, M., Braithwaite, M., De Souza, J. T., Jimenez-Bremont, J. F., Ohkura, M., Stewart, A., & Mendoza-Mendoza, A. (2017). Environmental growth conditions of Trichoderma spp. Affects indole acetic acid derivatives, volatile organic compounds, and plant growth promotion. Frontiers in Plant Science, 8(February), 1–18. Retrieved from: https://doi.org/10.3389/fpls.2017.00102.
Nuzullah, A. F., & Firgiyanto, R. (2021). Aplikasi berbagai jenis media dan ZPT terhadap aklimatisasi anggrek Vanda (Vanda sp.). Agropross: National Conference Proceedings of Agriculture, 5(2021), 10–24. Retrieved from: https://doi.org/10.25047/agropross.2021.202.
Patavardhan, S. S., Ignatius, S., Thiyam, R., Lasrado, Q., Karkala, S., D’Souza, L., & Nivas, S. K. (2022). Asymbiotic seed germination and in vitro development of orchid Papilionanthe Miss Joaquim. Ornamental Horticulture, 28(2), 246–255. Retrieved from: https://doi.org/10.1590/2447-536X.v28i2.2431.
Sparta, A., & Emilda, D. (2020). Growth Evaluation of banana cv. Barangan as the effect of Trichoderma sp. and covering types during acclimatization process. Caraka Tani: Journal of Sustainable Agriculture, 35(2), 268-277. Retrieved from: https://doi.org/10.20961/carakatani.v35i2.41191.
Sutarman, S., & Prahasti, T. (2022). Uji keragaan Trichoderma sebagai pupuk hayati dalam meningkatkan pertumbuhan dan produksi tanaman bawang merah. Jurnal Agrotek Tropika, 10(3), 421-428. Retrieved from: https://doi.org/10.23960/jat.v10i3.5737.
Syamsuri, R. R. P., Aprilia, D. A., Fakhira, A. Y., Nabilah, A. S., Akbari, S. I., Rossiana, N., & Doni, F. (2022). Prospecting the roles of Trichoderma in sustainable crop production: biotechnological developments and future prospects. Bioscience, 6(2), 101-116. Retrieved from: https://doi.org/10.24036/0202262119346-0-00.
Thomas, J., Ajay, D., Kumar, R. R., & Mandal, A. K. A. (2010). Influence of beneficial microorganisms during in vivo acclimatization of in vitro-derived tea (Camellia sinensis) plants. Plant Cell, Tissue and Organ Culture, 101(3), 365–370. Retrieved from: https://doi.org/10.1007/s11240-010-9687-7.
Vinod-Kumar, N., Subha Rajam, K., & Esther Rani, M. (2017). Plant growth promotion efficacy of Indole Acetic Acid (IAA) produced by a mangrove associated fungi-Trichoderma viride VKF3. International Journal of Current Microbiology and Applied Sciences, 6(11), 2692–2701. Retrieved from: https://doi.org/10.20546/ijcmas.2017.611.317.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Zulfa Ulinnuha, Noor Farid
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be transferred to AGROSCRIPT Journal of Applied Agricultural Sciences
This is an open-access journal in accordance with the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license.